Pouring SDS PAGE gel
This video shows how to pour an poly-acrylamide gel for a SDS PAGE protein in preparation for a western blot analysis.
Description
Non-interactive video download link (mp4).
The script that has been used to create the video shown above can be downloaded as excel script file.
1 | The polyacrylamide-gels are cast for you. The assistant will give them to you. |
2 | Think about how to load the gels; What is a logical order? Let your order checked by one of the assistants. |
After waiting long enough you try to remove the gel but it has not polymerized well. What went wrong?
- Too much APS and TEMED was added
Correct = no - Too little APS and TEMED was added
Correct = yes - You waited too long before removing the gel
Correct = no - The glass-plate sandwich was not properly clean before use
Correct = no
Feedback if a or c: Incorrect. This will not have an effect on the polymerization of the gel.
Feedback if correct: Correct! With too little APS and/or TEMED the gel is not able to polymerize properly and the gel will stay liquid.
Feedback if d: Incorrect. This will not have an effect on the polymerization of the gel. It can however contaminate your gel.
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- Skill levelIntroduction video
- CategoryBiochemistry
