The script that has been used to create the video shown above can be downloaded as excel script file.
Now we have the blocking buffer, we need to transfer the membrane in to a 50 ml tube.
What is the correct way to place the PVDF membrane into the tube for blocking?
Before we can block our membrane, we need to prepare blocking buffer.
We will make 50 ml of blocking buffer. Calculate how many grams of non-fat dry milk we need to add to TBS-T to make a 5% solution.
We need ____ g of non-fat dry milk
Correct answer: 2.5
De blokbuffer bevat melkpoeder. Waarvoor dient dit?
Blocking your membrane is a very important step of Western blotting. But what happens during this step? Wich of the following statements is/are true?