Solid phase extraction (interactive)

Solid phase extraction is a sample cleanup method which can be adapted to almost any analyte, simply by changing the stationary phase of the SPE cartridge. In this video, a reverse phase cartridge is used to retain parabens from an aqueous sample. By adjusting pH and using various solvents, the parabens are then eluted in acetonitrile for downstream analysis. This video was recorded to introduce students to SPE cartridges, vacuum manifolds, and to demonstrate proper techniques.

• Tineke Bijl
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