Text of: Parabens: what solvent for eluting

In the lab reverse-phase (RP) SPE cartridges will be available.

What solvent would you like to use to elute the analytes from the SPE column?

Solvent:

• Group: 2, identifier: x2
  …
• Group: 2, identifier: e2
  acetonitrile
• Group: 2, identifier: a2
  deionized water
• Group: 2, identifier: d2
  methanol
• Group: 2, identifier: h2
  tetrahydrofuran
• Group: 2, identifier: f2
  water (pH 3)
• Group: 2, identifier: g2
  water (pH 7)

• Mapping, Correct=0
  • Match solvent against x2
  • Fill in all the gaps!
• Mapping, Correct=0
  • OR
    • Match solvent against g2
    • Match solvent against a2
    • Match solvent against f2
  • The purpose of this step is to elute the hydrophobic analytes from the column material. How can you achieve this with a hydrophilic solvent?
• Mapping, Correct=0
  OR
  • • Match solvent against h2
  • Tetrahydrofuran would be suitable because it is apolar. However, it is quite toxic. Avoid the use of toxic substances if good alternatives are available.
• Mapping, Correct=1
  Match solvent against e2
  • You have chosen to elute the analytes from the column with acetonitrile.
• Mapping, Correct=1
  Match solvent against d2
  • You have chosen to elute the analytes from the column with methanol.